abstract：:The thermal stability and solubility profiles of human hypertrophic scar collagen have been measured and compared with those of the collagens present in human normal scar, human normal skin and a lathyritic dermal tissue. The thermal stability profiles of the hypertrophic scar collagens were found to be very similar t...

journal_title：Connective tissue research

authors： Snowden JM,Swann DA

更新日期：1984-01-01 00:00:00

abstract：:Heparin treated and aldehyde crosslinked rat aorta segments were implanted in infrarenal aorta of homologous rats. One year following aortic replacement, the subendothelial scar and the prosthetic remnants were excised. The scar and the host intima-media were incubated with 3H-valine for 4 h and extracted with 5 M gua...

journal_title：Connective tissue research

authors： Moczar M,Bessou JP,Loisance D

更新日期：1983-01-01 00:00:00

abstract：:We examined the effects of several variables on the ratio of type I:type III collagen secreted by human caucasian skin fibroblasts in normal and osteogenesis imperfecta (OI) phenotypes. Isotopically labelled collagen extracted from fibroblast medium was analyzed by DEAE-cellulose chromatography and identified by appro...

journal_title：Connective tissue research

authors： Fraser J,Lancaster GA,Scriver CR

更新日期：1983-01-01 00:00:00

abstract：:To extend previous studies on the apparent acceleration of collagen aging in diabetes mellitus, dura collagen from human adults of different ages, with and without diabetes, was processed to yield soluble and insoluble fractions. Insoluble fractions were cleaved by cyanogen bromide. Release of peptides from insoluble ...

journal_title：Connective tissue research

authors： Kohn RR

更新日期：1983-01-01 00:00:00

abstract：:The structural and immunological properties of the glycosaminoglycans and the core proteins of bovine nasal cartilage proteoglycan and the proteoglycans produced by rabbit articular chondrocytes in spinner and monolayer culture were compared. Culture medium with 35SO4- or 3H-serine-labeled proteoglycan was mixed with ...

journal_title：Connective tissue research

authors： Keiser HD,Malemud CJ

更新日期：1983-01-01 00:00:00

abstract：:Parietal yolk sacs isolated from 14.5-day rat embryos and incubated in vitro with either [14C]proline, [3H]mannose or 3H-labeled amino acid mixture synthesized and secreted basement membrane collagenous and noncollagenous glycoprotein components with relative molecular weights of 350,000 (350K), 220,000 (220K), 185,00...

journal_title：Connective tissue research

authors： Clark CC,Kefalides NA

更新日期：1982-01-01 00:00:00

abstract：:The total and individual glycosaminoglycan (GAG) content at various stages of the Dupuytren disease process and in samples of normal palmar connective tissue (palmar dermis, palmar fascia and digital flexor tendon) from the hands of uninvolved age-matched controls have been assayed and compared. Morphological comparis...

journal_title：Connective tissue research

authors： Flint MH,Gillard GC,Reilly HC

更新日期：1982-01-01 00:00:00

abstract：:Glycosaminoglycan synthesis by cells cultured from nodule or band tissues of patients with Dupuytren's disease was compared with that of skin fibroblasts cultured from uninvolved areas of the palm. No differences were observed in culture which could account either for the abnormal glycosaminoglycan content or the incr...

journal_title：Connective tissue research

authors： Slack C,Flint MH,Thompson BM

更新日期：1982-01-01 00:00:00

abstract：:To study the glycosylation of glomerular basement membrane collagen (GBMC) in diabetes, kidneys were obtained at autopsy from 5 patients with insulin-requiring diabetes of long duration and diabetic complications, and from 5 control subjects. Glomeruli were prepared by sieving and collagen was isolated by limited peps...

journal_title：Connective tissue research

authors： Uitto J,Perejda AJ,Grant GA,Rowold EA,Kilo C,Williamson JR

更新日期：1982-01-01 00:00:00

abstract：:A method is described for accurate and reproducible determination of wet and dry weights of small articular cartilage samples. The water content of the articular cartilage samples before and after treatment with selective degradative enzymes and "artificial fibrillation" was determined. It was found that the water con...

journal_title：Connective tissue research

authors： Simon WH,Wohl DL

更新日期：1982-01-01 00:00:00

abstract：:The oxidation of an elastin substrate by highly purified bovine aortic lysyl oxidase (LO) is markedly influenced by amphiphilic molecules known to bind to elastin. Negatively charged elastin ligands, including fatty acids, bile salts or sodium dodecyl sulfate can completely inhibit the oxidation of lysine in elastin, ...

journal_title：Connective tissue research

authors： Kagan HM,Simpson DE,Tseng L

更新日期：1981-01-01 00:00:00

abstract：:The connective tissue protein elastin is largely responsible for maintaining the elasticity of major blood vessels and lung tissue. Comparatively few studies have been made of the immunologic properties of elastin because of its high degree of insolubility and because of its apparent limited capacity to elicit precipi...

journal_title：Connective tissue research

authors： Kucich U,Christner P,Rosenbloom J,Weinbaum G

更新日期：1981-01-01 00:00:00

abstract：:Cell cultures propagated from fetal bovine ligamentum nuchae were found to accumulate an extensive extracellular matrix containing collagen and elastic fibers when maintained for periods up to 8 weeks. The synthesis by these cells of two glycoproteins, designated MFP I and MFP II, which are immunologically related to ...

journal_title：Connective tissue research

authors： Sear CH,Jones CJ,Knight KR,Grant ME

更新日期：1981-01-01 00:00:00

abstract：:A homogeneous preparation of glycoprotein (apparent molecular weight 34,000) has been isolated from bovine ligamentum nuchae by treatment of the tissue with 5 M guanidine HCl and 2-mercaptoethanol. The preparation exhibits amine oxidase activity which is only marginally inhibited by beta-aminopropionitrile. ...

journal_title：Connective tissue research

authors： Serafini-Fracassini A,Ventrella G,Griffiths R,Hinnie J

更新日期：1981-01-01 00:00:00

abstract：:X-Ray diffraction and scanning electron microscopy show that collagen fibrils tend to be oriented parallel to the surface of the vertebral end plates in pig, rat and man. It is suggested that this orientation allows the end plate to withstand the pressure exerted on it by the nucleus pulposus. Scanning electron micros...

journal_title：Connective tissue research

authors： Aspden RM,Hickey DS,Hukins DW

更新日期：1981-01-01 00:00:00

abstract：:Positive effects on the tensile characteristics of swine digital extensors were found following twelve months of exercise training. Compared to sedentary controls, the tendons from the exercised animals became stronger as a material and exhibited hypertrophy. These biomechanical results were supported by biochemical a...

journal_title：Connective tissue research

authors： Woo SL,Ritter MA,Amiel D,Sanders TM,Gomez MA,Kuei SC,Garfin SR,Akeson WH

更新日期：1980-01-01 00:00:00

abstract：:Elastic ear cartilage and aortae from nine-day-old hamsters were incubated in Krebs-Ringer medium containing ascorbate, beta-aminopropionitrile and either [14C]proline or [3H]valine. The [14C]proline-labeled proteins were separated by chromatography on Agarose A-5 m in sodium dodecyl sulfate and their [14C]-hydroxypro...

journal_title：Connective tissue research

authors： Heeger P,Rosenbloom J

更新日期：1980-01-01 00:00:00

abstract：:Hall type voltage has been observed in bone and its two major components (collagen and apatite) in the region of moderately high electric and magnetic fields. The configuration of the sample placement and the experimental set up was the same as that adopted in standard Hall measurements. The resistivity of the sample ...

journal_title：Connective tissue research

authors： Behari J,Andrabi WH

更新日期：1978-01-01 00:00:00

abstract：:Abnormal polyanion metabolism in cultured fibroblasts from patients with pseudoxanthoma elasticum (PXE) was studied by incubating normal and PXE fibroblasts in culture medium containing 35SO4 for 96 hr and measuring differences in secreted 35SO4-labelled proteoglycans (35S-PG). PXE medium contained less high molecular...

journal_title：Connective tissue research

authors： Gordon SG,Overland M,Foley J

更新日期：1978-01-01 00:00:00

abstract：:The effect of I- and C1- on the enzymatic degradation of elastin pancreatic elastase has been studied. At low halide concentrations both ions show a marked stimulation of elastolysis, I- being significantly more effective than Cl-. Kinetic plots of enzyme activity indicate that anions enhance the susceptibility of the...

journal_title：Connective tissue research

authors： Winkler RH

更新日期：1978-01-01 00:00:00

abstract：:The interaction of sodium dodecyl sulfate with elastin has been studied by complexometric titration. Approximately 1.2 mumoles of protons with a pKapp of 5.45 are taken up by 10 milligrams of insoluble elastin upon the binding of detergent, apparently due to the protonation of normally ionized carboxylate functions in...

journal_title：Connective tissue research

authors： Kagan HM

更新日期：1978-01-01 00:00:00

abstract：:Short term cultures were carried out with chondrocytes and tissue fragments from fetal guinea pig epiphyseal cartilage. Proteoglycans were isolated from these cultures and their properties were compared with those of proteoglycans from adult hyaline cartilage. It was concluded that the proteoglycans synthesized in cul...

journal_title：Connective tissue research

authors： Madsen K,Lohmander S,Moskalewski S,Hinek A

更新日期：1978-01-01 00:00:00

abstract：:The use of electron microscopic observations to characterize the state of aggregation of collagen molecules in solution has been examined from the point of view that the observed structures must be compatible with the physical chemical properties of the solution. The common procedure of addition of droplets of a colla...

journal_title：Connective tissue research

authors： Schwartz D,Veis A

更新日期：1978-01-01 00:00:00

abstract：:Scanning electron microscopy and high angle X-ray diffraction were used to define the relationship between collagen and elastin of bovine aortic wall. The diffraction pattern shows on one hand that the broad rings at 4.5 A and 9 A, due to elastin, do not orient on stretching and on the other hand, that the collagen ri...

journal_title：Connective tissue research

authors： Bigi A,Ripamonti A,Roveri N

更新日期：1977-01-01 00:00:00

abstract：:The basic subunit of cartilage proteoglycan consists of multiple glycosaminoglycan chains covalently attached to a core protein. It is unclear as to whether there is a single core protein or multiple different core proteins, since previous studies using either chondroitinase or testicular hyaluronidase to enzymaticall...

journal_title：Connective tissue research

authors： Keiser HD,Hatcher VB

更新日期：1977-01-01 00:00:00

abstract：:The effects of two forms of elastase on the Young's Modulus of elastic tissue have been studied. The monomeric, calcium poor form of the enzyme lowers the value of the Young's Modulus before an appreciable amount of protein is taken into solution. The polymeric, calcium rich enzyme on the other hand degrades the prote...

journal_title：Connective tissue research

authors： Hall DA

更新日期：1976-01-01 00:00:00

abstract：:DNA synthesis in rat and rabbit polyvinyl sponge induced granulation tissue has been studied using thymidine (methyl-3H). Synthesis was determined by measurement of thymidine incorporation into cold trichloroacetic acid insoluble material and by autoradiography. Granulation tissue was removed and immediately incubated...

journal_title：Connective tissue research

authors： Wiener SL,Urivetzky M,Isenberg HD,Havier R,Wiener R,Belenko M,Heydu E,Meilman E

更新日期：1976-01-01 00:00:00

abstract：:Hyaluronate lyase from streptococci group A was purified by chromatography on DEAE-Sephadex A-50 and on Biogel P-150, thereby enriching it about 1,000-fold and separating it into two enzyme fractions with the same amino acid composition. The photooxidation of hyaluronate lyase in the presence of methylene blue results...

journal_title：Connective tissue research

authors： Greiling H,Stuhlsatz HW,Eberhard T,Eberhard A

更新日期：1975-01-01 00:00:00

abstract：:Depolymerization of Ch-4-S, Ch-6-S, HA and HP was observed by gel filtration after treatment with 2-10 N NaOH or Ba(OH)2 in the presence of NaBH4 at 80-83 degrees for 1-5 hr. Depolymerized products were isolated after these treatments. 4-Deoxy-alpha-L-threo-4-enohexopyranosyluronic acid residue as the nonreducing end ...

journal_title：Connective tissue research

authors： Hirano S,Ishigami M,Koga Y

更新日期：1975-01-01 00:00:00